ABSTRACT

Automated DNA Typing: Method of the Future?   [open pdf - 22KB]

"Although highly reliable in clinical or research applications, the standard technology used for DNA typing - known as RFLP-VNTR analysis - has been less satisfactory in the forensic setting. RFLP-VNTR requires abundant and clean specimens; samples typically found at crime scenes, however, are both quantitatively and qualitatively inadequate - very small and often environmentally degraded from exposure to heat, light, and humidity. Further, police investigations call for a quicker turnaround time than is possible with the standard method, which can take months to complete. An NIJ-sponsored project at Baylor University's College of Medicine sought a DNA typing system that overcomes the limitations of samples found at crime scenes. The project replicated the DNA sample (i.e., synthesized new DNA from existing DNA) to obtain sufficient quantities for analysis and then identified genetic markers for DNA typing. That procedure, known as PCR-STR,3 can produce reliable results with degraded specimens, is quick, and can be automated to permit the creation of a vastly improved data base of DNA profiles of convicted offenders. PCR-STR promises to extend the application of DNA typing as a powerful criminal justice tool that helps to establish, with a high degree of certitude, the guilt or innocence of suspects."

Publisher:
Date:
1997-02
Copyright:
Public Domain
Retrieved From:
National Criminal Justice Reference Service: http://www.ncjrs.gov
Format:
pdf
Media Type:
application/pdf
URL:
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